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Species: Mus musculus
Genetic Insert: mHIF-1α (P402A/P577A/N813A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-1α.
Full-length murine HIF-1α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-1α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 402, Proline 577 and Asparagine 813 to alanines. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
Alternate plasmid names: HIF-1α triple mutant (TM); pcDNA3 HIF-1α TM
Proper citation: RRID:Addgene_44028 Copy
Species: Mus musculus
Genetic Insert: Pomc promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequence shows a C->T mutation in nt 129 of POMC. The depositing scientist is aware of this mutation and has found that it does not alter the promoter activity.
Proper citation: RRID:Addgene_17553 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The mouse c-myc cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the original vector with mouse c-myc.
Proper citation: RRID:Addgene_36980 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_154106 Copy
Species: Homo sapiens
Genetic Insert: Tissue factor promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: -111 to +14 hTF promoter with all 3 EGR-1 sites mutated. hTF promoter deletion/mutation series 2, Mackman lab #2447.
Proper citation: RRID:Addgene_15450 Copy
Species: Homo sapiens
Genetic Insert: Ubiquilin 4
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCGGAGCCGAGCGG-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GGAGAGCTGGGAGCCCAG-3'.
Proper citation: RRID:Addgene_16170 Copy
Species: Gallus gallus
Genetic Insert: shh
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_13991 Copy
Species: Homo sapiens
Genetic Insert: PAK5
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Order of elements: SalI-Myc-XbaI-Pak5-EcoRI
Proper citation: RRID:Addgene_16020 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:https://www.nature.com/articles/nbt1036; Vector Backbone:pFBDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160451 Copy
Species: Homo sapiens
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16061 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Dominate Negative form of TRF2
Proper citation: RRID:Addgene_16069 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16067 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_159298 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_159297 Copy
Species: Homo sapiens
Genetic Insert: HtrA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This construct was used in the experiments described in figure 5
Proper citation: RRID:Addgene_15938 Copy
Species: Mus musculus
Genetic Insert: SRY-box containing gene 2
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_15919 Copy
Species: Other
Genetic Insert: mFAP10
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159463 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15974 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pFA6a-5FLAG-KanMX6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Template vector for a PCR based gene-tagging (5 x FLAG tag) method in S. pombe.
Proper citation: RRID:Addgene_15983 Copy
Species: Rattus norvegicus
Genetic Insert: AMPK alpha2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15992 Copy
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