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Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99679 Copy
Species: Other
Genetic Insert: Galdieria sulphuraria PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166419 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166418 Copy
Species: Mus musculus
Genetic Insert: anti-FGF13/FHF2, B isoform (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2916243. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_188208 Copy
Species:
Genetic Insert: SARS-CoV-2 BA.2.75 Spike
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_190012 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99680 Copy
Species: Synthetic
Genetic Insert: Luc-E2A-mCherry-T2A-PuroR
Vector Backbone Description: Backbone Marker:I. Verma, Salk; Backbone Size:9221; Vector Backbone:p.FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183685 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99685 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99687 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99686 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99689 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAAV (pUC f1); Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99688 Copy
Species: Mus musculus
Genetic Insert: Adar2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9175; Vector Backbone:pSpCas9(BB)-2A-Puro PX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2022.07.12.499727 for bioRxiv preprint.
Proper citation: RRID:Addgene_158118 Copy
Species: Homo sapiens
Genetic Insert: P62
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pET-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: https://hub.asap.science/shared-research/1823e9d4-44a1-4cc7-9cc7-f9557ff1f152
Proper citation: RRID:Addgene_190929 Copy
Species: Mus musculus
Genetic Insert: Adar2
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9175; Vector Backbone:pSpCas9(BB)-2A-Puro PX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2022.07.12.499727 for bioRxiv preprint.
Proper citation: RRID:Addgene_158119 Copy
Species: Homo sapiens
Genetic Insert: NEK1
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189871 Copy
Species: Other
Genetic Insert: Naegleria fowleri CBS-pair domain
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166437 Copy
Species: Homo sapiens
Genetic Insert: NEK5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189876 Copy
Species: Caenorhabditis elegans
Genetic Insert: Caenorhabditis elegans CNNM5 CBS-pair domain
Vector Backbone Description: Backbone Marker:Amersham-Pharmacia; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166434 Copy
Species: Other
Genetic Insert: CsChrimson
Vector Backbone Description: Backbone Marker:Olena Riabinina, Darya Task, Elizabeth Marr, Chun-Chieh Lin, Robert Alford, David A. O'Brochta & Christopher J. Potter; Backbone Size:6637; Vector Backbone:pXL-BacII; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_175548 Copy
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