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Species: Other
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pMT85-2res; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_173895 Copy
Species: Homo sapiens
Genetic Insert: human full-length CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_109156 Copy
Species: Homo sapiens
Genetic Insert: human C-terminal CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_109159 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtDREB26_HiBit
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Other, Gateway entry vector ; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
Proper citation: RRID:Addgene_196145 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTGA2_HiBit
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Other, Gateway entry vector ; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
Proper citation: RRID:Addgene_196142 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTGA1_HiBit
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Other, Gateway entry vector ; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
Proper citation: RRID:Addgene_196141 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtNLP7_HiBit
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Other, Gateway entry vector ; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
Proper citation: RRID:Addgene_196144 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Other, Diatom Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_188609 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_188610 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2607; Vector Backbone:BASIC_SEVA_67.10; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178940 Copy
Species: E.coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14467 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F.
Proper citation: RRID:Addgene_158628 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
Proper citation: RRID:Addgene_158633 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
Proper citation: RRID:Addgene_158630 Copy
Species: Other
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167131 Copy
Species: Other
Genetic Insert: SARS-CoV-2 3a protein (aa 42-275)
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:Derived from pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Truncation made by PCR.
Please visit https://www.biorxiv.org/lookup/doi/10.1101/2020.06.17.156554 for bioRxiv preprint.
Proper citation: RRID:Addgene_156425 Copy
Species: Other
Genetic Insert: ID#74
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Other, pENTRY clone; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175929 Copy
Species: Other
Genetic Insert: ID#45
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Other, pENTRY clone; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175920 Copy
Species: Other
Genetic Insert: ID#55
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Other, pENTRY clone; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175921 Copy
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