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Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4445; Vector Backbone:JK104; Vector Types:Mammalian Expression, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121858 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:2677; Vector Backbone:IR301; Vector Types:Mammalian Expression, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121851 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4120; Vector Backbone:JK104; Vector Types:Mammalian Expression, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121853 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:32643; Vector Backbone:IQ013; Vector Types:Adenoviral, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121844 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:32694; Vector Backbone:IQ013; Vector Types:Adenoviral, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121846 Copy
Species: Synthetic
Genetic Insert: 3x HA epitope tag-PolyA + hU6-xCas9(3.7) gRNA scaffold
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMAGIC Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. 3x HA tag + bGH polyA; hU6 promoter PCR'd from Addgene 61591. x/SpCas9 gRNA scaffold derived from PMID: 26671237
Proper citation: RRID:Addgene_121842 Copy
Species: Synthetic
Genetic Insert: AkCas12b
Vector Backbone Description: Vector Backbone:BPK2013-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_121950 Copy
Species: Synthetic
Genetic Insert: BsCas12b
Vector Backbone Description: Vector Backbone:BPK2013-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_121952 Copy
Species: Synthetic
Genetic Insert: AaCas12b
Vector Backbone Description: Vector Backbone:BPK2014-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_121949 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4682; Vector Backbone:KX002; Vector Types:Mammalian Expression, Other, PiggyBac Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: PiggyBac ITR sequences derived from Addgene #63802
Proper citation: RRID:Addgene_121871 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:5007; Vector Backbone:KX002; Vector Types:Mammalian Expression, Other, PiggyBac Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: PiggyBac ITR sequences derived from Addgene #63807
Proper citation: RRID:Addgene_121876 Copy
Species: Synthetic
Genetic Insert: ASAP1
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other, Tol2 transposon destination vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121987 Copy
Species: Synthetic
Genetic Insert: AkCas12b single chimeric gRNA
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121955 Copy
Species: Synthetic
Genetic Insert: AmCas12b single chimeric gRNA
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121956 Copy
Species: Synthetic
Genetic Insert: AaCas12b single chimeric gRNA, MS2 hairpin inserted
Vector Backbone Description: Vector Backbone:pUC19, BsaI site mutated; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121959 Copy
Species: Synthetic
Genetic Insert: rsCaMPARI
Vector Backbone Description: Backbone Size:4270; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122092 Copy
Species: Synthetic
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122099 Copy
Species: Synthetic
Genetic Insert: NmCas9 tracrRNA
Vector Backbone Description: Backbone Size:4314; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122091 Copy
Species: Synthetic
Genetic Insert: SpCas9 tracr
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122089 Copy
Species: Synthetic
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4974; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122064 Copy
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