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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMV1-SMAC-HA-eGFP Resource Report Resource Website |
RRID:Addgene_67487 | SMAC | Homo sapiens | Ampicillin | PMID:27057421 | Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186. | Vector Backbone:pCMV(delta1); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa1-59 | 2024-08-01 01:09:26 | 0 |
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pCMV2-SMAC-HA-eGFP Resource Report Resource Website |
RRID:Addgene_67488 | SMAC | Homo sapiens | Ampicillin | PMID:27057421 | Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186. | Vector Backbone:pCMV(delta2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa1-59 | 2024-08-01 01:09:26 | 0 |
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pCMV6-OCT-HA-eGFP Resource Report Resource Website |
RRID:Addgene_67485 | OCT-HA-eGFP | Homo sapiens | Ampicillin | PMID:27057421 | Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348 | Vector Backbone:pCMV(delta6); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:09:26 | 0 | |
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SND1 (SND1A-c003) Resource Report Resource Website |
RRID:Addgene_98242 | SND1A | Homo sapiens | Kanamycin | PMID: | Use BL21(DE3)-R3-pRARE2 for expression. T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. 5M9O: http://www.thesgc.org/structures/5M9O | Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | pfam00567:TUDOR 677-799 | 2024-08-01 01:10:41 | 0 |
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pGEX6P1-Ubl Resource Report Resource Website |
RRID:Addgene_78712 | Human Sacsin 2-85 | Homo sapiens | Ampicillin | PMID:29945973 | Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2024-08-01 01:09:58 | 0 | ||
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pTag-RFP-C-h-Rab11a Resource Report Resource Website |
RRID:Addgene_79806 | Rab11a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2024-08-01 01:10:01 | 0 | ||
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pCMV6-SMAC-HA-eGFP Resource Report Resource Website |
RRID:Addgene_67492 | SMAC | Homo sapiens | Ampicillin | PMID:27057421 | Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186. | Vector Backbone:pCMV(delta6); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa1-59 | 2024-08-01 01:09:26 | 0 |
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pCMV5-SMAC-HA-eGFP Resource Report Resource Website |
RRID:Addgene_67491 | SMAC | Homo sapiens | Ampicillin | PMID:27057421 | Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186. | Vector Backbone:pCMV(delta5); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa1-59 | 2024-08-01 01:09:26 | 0 |
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pTag-BFP-C-h-Rab11a Resource Report Resource Website |
RRID:Addgene_79805 | Rab11a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2024-08-01 01:10:01 | 0 | ||
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pPN130 Resource Report Resource Website |
RRID:Addgene_91637 | MAD1L1 | Homo sapiens | Ampicillin | PMID:29020615 | Vector Backbone:pUC19-U6 promoter; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2024-08-01 01:10:36 | 0 | ||
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pPN131 Resource Report Resource Website |
RRID:Addgene_91638 | MAD1L1 | Homo sapiens | Ampicillin | PMID:29020615 | Vector Backbone:pUC19-U6 promoter; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2024-08-01 01:10:36 | 0 | ||
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pET21 MBPx-SpyCatcher Resource Report Resource Website |
RRID:Addgene_72327 | pET21 MBPx-SpyCatcher | Synthetic | Ampicillin | PMID:26787909 | Addgene QC NGS analysis identifies a deletion of one nucleotide in the lacI sequence, relative to the expected backbone sequence. This would result in a truncation of the expected protein. The depositing laboratory has confirmed that this is of no substantial consequence. | Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | MBP contains A312V, I317V mutations and 172, 173, 175 and 176 deletions | 2024-08-01 01:09:38 | 0 |
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pEGFP-rSyntenin1 Resource Report Resource Website |
RRID:Addgene_89431 | SDCBP | Rattus norvegicus | Kanamycin | PMID:11152476 | Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2024-08-01 01:10:29 | 0 | ||
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pEGFP-hSyntenin2a Resource Report Resource Website |
RRID:Addgene_89436 | SDCBP2 | Homo sapiens | Kanamycin | PMID:11152476 | Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2024-08-01 01:10:29 | 0 | ||
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p663-UBC-miniTurbo-V5-WEE2_IDG-K Resource Report Resource Website |
RRID:Addgene_189895 | WEE2 | Homo sapiens | Ampicillin | PMID: | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2024-08-01 01:05:14 | 0 | |
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pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif) Resource Report Resource Website |
RRID:Addgene_166415 | Acytostelium subglobosum PRL | Other | Ampicillin | PMID:36822330 | Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Deleted nucleotides coding for 4 C-terminal amino acids | 2024-08-01 01:04:00 | 0 | |
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dSp-VP64-p65(100-261)-RTA(75-190) Resource Report Resource Website |
RRID:Addgene_99672 | dCas9 | Synthetic | Ampicillin | PMID: | Please visit https://doi.org/10.1101/298620 for bioRxiv preprint. | Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | dead Cas9 | 2024-08-01 01:10:45 | 0 |
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dSp-VP64-p65(150-261)-RTA(125-190) Resource Report Resource Website |
RRID:Addgene_99674 | dCas9 | Synthetic | Ampicillin | PMID: | Please visit https://doi.org/10.1101/298620 for bioRxiv preprint. | Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | dead Cas9 | 2024-08-01 01:10:45 | 0 |
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pET-11-LECT2-V40 Resource Report Resource Website |
RRID:Addgene_183031 | LECT2 | Homo sapiens | Ampicillin | PMID: | Please visit https://doi.org/10.1101/2022.03.01.482540 for bioRxiv preprint. | Backbone Marker:Novagen/EMD; Backbone Size:5677; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | V40 variant | 2024-08-01 01:04:52 | 0 |
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dSp-VP64-RTA Resource Report Resource Website |
RRID:Addgene_99675 | dCas9 | Synthetic | Ampicillin | PMID: | Please visit https://doi.org/10.1101/298620 for bioRxiv preprint. | Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | dead Cas9 | 2024-08-01 01:10:45 | 0 |
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