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Species: Synthetic
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: Luc2 PCR'd from pGL4.23 (Promega)
Proper citation: RRID:Addgene_121741 Copy
Species: Other
Genetic Insert: osTir1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: osTir1/9xmyc sequence PCR'd from Addgene #47328
Proper citation: RRID:Addgene_121742 Copy
Species: Homo sapiens
Genetic Insert: Human PDX1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: PDX1 PCR'd from HsCD00082591 (DNASU)
Proper citation: RRID:Addgene_121744 Copy
Species: Synthetic
Genetic Insert: βgal
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121738 Copy
Species: Synthetic
Genetic Insert: βgal (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121739 Copy
Species: Synthetic
Genetic Insert: cTnT promoter (Cardiomyocyte specific)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221 P1-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121690 Copy
Species: Synthetic
Genetic Insert: CMV promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121692 Copy
Species: Synthetic
Genetic Insert: EF1a promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121693 Copy
Species: Synthetic
Genetic Insert: cTnT promoter (Cardiomyocyte specific)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121696 Copy
Species: Synthetic
Genetic Insert: HA epitope tag (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121699 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121732 Copy
Species: Synthetic
Genetic Insert: mCherry (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121733 Copy
Species: Synthetic
Genetic Insert: RIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121728 Copy
Species: Synthetic
Genetic Insert: TRE promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TRE promoter sequence PCR'd from PTight promoter (Clontech)
Proper citation: RRID:Addgene_121729 Copy
Species: Synthetic
Genetic Insert: TBG promoter (Liver specific)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221 P1-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TBG promoter sequence PCR'd from Addgene #61593
Proper citation: RRID:Addgene_121689 Copy
Species: Synthetic
Genetic Insert: osTir1-P2A-AID (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: osTir1/9xmyc sequence PCR'd from Addgene #47328. Minimal auxin-inducible degron (AID) sequence derived from PMID: 27052166.
Proper citation: RRID:Addgene_121722 Copy
Species: Synthetic
Genetic Insert: Blast-P2A (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121715 Copy
Species: Synthetic
Genetic Insert: Neo-P2A (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121717 Copy
Species: Synthetic
Genetic Insert: Hygro-P2A (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121718 Copy
Species: Synthetic
Genetic Insert: Zeo-P2A (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2554; Vector Backbone:pDONR221 P5-P4; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121719 Copy
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