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Species:
Genetic Insert: YFP
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Other, Broad-host range; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_90104 Copy
Species: Homo sapiens
Genetic Insert: FIP200
Vector Backbone Description: Backbone Marker:Protech Facilities VBCF, Vienna, Austria; Backbone Size:2935; Vector Backbone:pGB-02-03; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_187832 Copy
Species: Other
Genetic Insert: mCh-V5-NES-EGFP-mCBP80
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192313 Copy
Species: Other
Genetic Insert: pLacZ-ECFP in pBBR1-mcs5
Vector Backbone Description: Backbone Size:800; Vector Backbone:pBBR1-mcs5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: ECFP from Clontech
Proper citation: RRID:Addgene_107775 Copy
Species:
Genetic Insert: TevSpCas9, sgRNA cassette, oriT, CenArsHis
Vector Backbone Description: Backbone Size:52707; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_202796 Copy
Species: Other
Genetic Insert: Lifeact-Citrine
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Other; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_100590 Copy
Species: Homo sapiens
Genetic Insert: beta-actin
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_178076 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV SpCas9; Vector Types:Mammalian Expression, CRISPR, Other, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_206267 Copy
Species: Other
Genetic Insert: H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Other, Recombinant baculovirus production, CRE Acceptor (MultiBac); Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_206266 Copy
Species: Homo sapiens
Genetic Insert: H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Other, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
References:
Comments: L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.
Proper citation: RRID:Addgene_206280 Copy
Species: Other
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_199241 Copy
Species: Homo sapiens
Genetic Insert: CENP-L and His-CENP-N
Vector Backbone Description: Vector Backbone:pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_134205 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
Species: Homo sapiens
Genetic Insert: human full-length CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_109156 Copy
Species: Homo sapiens
Genetic Insert: human C-terminal CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_109159 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2607; Vector Backbone:BASIC_SEVA_67.10; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178940 Copy
Species: Other
Genetic Insert: SARS-CoV-2 3a protein (aa 42-275)
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:Derived from pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments: Truncation made by PCR.
Please visit https://www.biorxiv.org/lookup/doi/10.1101/2020.06.17.156554 for bioRxiv preprint.
Proper citation: RRID:Addgene_156425 Copy
Species: Other
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167131 Copy
Species: Other
Genetic Insert: ID#74
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Other, pENTRY clone; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175929 Copy
Species: Other
Genetic Insert: ID#45
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Other, pENTRY clone; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175920 Copy
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