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Species: Homo sapiens
Genetic Insert: SMAC
Vector Backbone Description: Vector Backbone:pCMV(delta1); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
Proper citation: RRID:Addgene_67487 Copy
Species: Homo sapiens
Genetic Insert: SMAC
Vector Backbone Description: Vector Backbone:pCMV(delta2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
Proper citation: RRID:Addgene_67488 Copy
Species: Homo sapiens
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta6); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67485 Copy
Species: Homo sapiens
Genetic Insert: SND1A
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Use BL21(DE3)-R3-pRARE2 for expression. T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. 5M9O: http://www.thesgc.org/structures/5M9O
Proper citation: RRID:Addgene_98242 Copy
Species: Homo sapiens
Genetic Insert: Human Sacsin 2-85
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_78712 Copy
Species: Homo sapiens
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_79806 Copy
Species: Homo sapiens
Genetic Insert: SMAC
Vector Backbone Description: Vector Backbone:pCMV(delta6); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
Proper citation: RRID:Addgene_67492 Copy
Species: Homo sapiens
Genetic Insert: SMAC
Vector Backbone Description: Vector Backbone:pCMV(delta5); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
Proper citation: RRID:Addgene_67491 Copy
Species: Homo sapiens
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_79805 Copy
Species: Homo sapiens
Genetic Insert: MAD1L1
Vector Backbone Description: Vector Backbone:pUC19-U6 promoter; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_91637 Copy
Species: Homo sapiens
Genetic Insert: MAD1L1
Vector Backbone Description: Vector Backbone:pUC19-U6 promoter; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_91638 Copy
Species: Synthetic
Genetic Insert: pET21 MBPx-SpyCatcher
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene QC NGS analysis identifies a deletion of one nucleotide in the lacI sequence, relative to the expected backbone sequence. This would result in a truncation of the expected protein. The depositing laboratory has confirmed that this is of no substantial consequence.
Proper citation: RRID:Addgene_72327 Copy
Species: Rattus norvegicus
Genetic Insert: SDCBP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_89431 Copy
Species: Homo sapiens
Genetic Insert: SDCBP2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_89436 Copy
Species: Homo sapiens
Genetic Insert: WEE2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189895 Copy
Species: Other
Genetic Insert: Acytostelium subglobosum PRL
Vector Backbone Description: Backbone Marker:Novagen Inc., Madison, WI; Backbone Size:5708; Vector Backbone:pET-15B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166415 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99672 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99674 Copy
Species: Homo sapiens
Genetic Insert: LECT2
Vector Backbone Description: Backbone Marker:Novagen/EMD; Backbone Size:5677; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2022.03.01.482540 for bioRxiv preprint.
Proper citation: RRID:Addgene_183031 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.
Proper citation: RRID:Addgene_99675 Copy
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