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Species: Xenopus laevis
Genetic Insert: Axin DeltaRGS
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: Dominant negative Xaxin. For sense transcripts, cut with NotI and transcribe with SP6.
To make antisense probe for whole mounts, cut with Pst1 and then remove 3' overhang before transcribing with T7.
Proper citation: RRID:Addgene_16304 Copy
Species: Xenopus laevis
Genetic Insert: Axin DeltaGID
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6.
GID= GSK3 interaction domain. NcoI digest should cut out a 1.733kb insert.
Please note that the GSK interaction domain deletion begins at aa 321 when the sequence is compared to GenBank entry NM_001088405.1. As a result of the cloning process, there are two amino acids 'HM' prior to the end of the deletion at aa 504 that are not part of the endogenous axin sequence.
Addgene's sequencing results also found a D559E mutation in the axin sequence that reflects a single nucleotide polymorphism. The depositing laboratory states that these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_16303 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Isolated from head library using xfz8 as a probe. Works well for in situs.
Proper citation: RRID:Addgene_16310 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See author's map for details. 1 kb EcoRI-ApaI plus 2.3 kb ApaI-SalI fragments of Xdsh cDNA were cloned into 4.3 kb EcoRI-XhoI cut pCS2-myc vector in-frame.
Proper citation: RRID:Addgene_15490 Copy
Species: Xenopus laevis
Genetic Insert: Centrin 4
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Other, reporter for basal body; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26753 Copy
Species: Xenopus laevis
Genetic Insert: lhx1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Mutated HNF1 binding site in lhx1 promoter: TcgAgGGTTTAC
Proper citation: RRID:Addgene_30493 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR1-ICD-FRB
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31261 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR4-ICD-Fv’Fv
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31258 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR1-ICD-Fv’Fv
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31257 Copy
Species: Xenopus laevis
Genetic Insert: Xenopus FGFR1-ICD-FKBP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31259 Copy
Species: Xenopus laevis
Genetic Insert: glycogen synthase kinase 3
Vector Backbone Description: Backbone Size:4096; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29679 Copy
Species: Xenopus laevis
Genetic Insert: dishevelled
Vector Backbone Description: Backbone Size:4096; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29683 Copy
Species: Xenopus laevis
Genetic Insert: Cerberus
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For injection linearize with NotI and transcribe with Sp6
Proper citation: RRID:Addgene_24679 Copy
Species: Xenopus laevis
Genetic Insert: Insulin-like growth factor 2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense linearize with NotI, transcribe with SP6.
For antisense, linearize with EcoRI, transcribe with T7.
Proper citation: RRID:Addgene_24689 Copy
Species: Xenopus laevis
Genetic Insert: xenopus paraxial protocadherin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS-SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: For antisense linearize with NotI
Transcribe with T7
Proper citation: RRID:Addgene_24687 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: HA tag: YPYDVPVYVL
silent mutation at (bp) 657 of szl
E248D does not affect function.
Proper citation: RRID:Addgene_24683 Copy
Species: Xenopus laevis
Genetic Insert: Chordin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Chordin expression vector, tagged. Insert of pSP35 Chd-Myc (Chd coding region). Insert cut from pSP35Chd-Myc (from a dcm-, dam- bacterial strain, as Xba I is there in a methylation consensus) with EcoRI and XbaI and subcloned in the same sites of pCS2.
For sense RNA injection: linearize with NotI
Transcribe with SP6
Proper citation: RRID:Addgene_24682 Copy
Species: Xenopus laevis
Genetic Insert: Dominant negative xIGFR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense, linearize with NotI, transcribe with SP6.
For antisense, linearize with EcoRI, transcribe with T7.
Proper citation: RRID:Addgene_24699 Copy
Species: Xenopus laevis
Genetic Insert: VSP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid encoding Xl-VSP1 (BC099069, clone # 6868954) was purchased from Open Biosystems (Huntsville, AL).
Proper citation: RRID:Addgene_51880 Copy
Species: Xenopus laevis
Genetic Insert: VSP2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Other, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid encoding Xl-VSP2 (CF520610, clone # 7008398) was purchased from Open Biosystems (Huntsville, AL).
Proper citation: RRID:Addgene_51885 Copy
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