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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126185 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126188 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126201 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3590; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Grow bacterial cultures in 20 micromolar Chloramphenicol. Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126200 Copy
Species:
Genetic Insert: BenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1
Vector Backbone Description: Vector Backbone:J64100; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please note there is a single mutation in arabinose V113A, and a single mutation in BxbI integrase E117G. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_126540 Copy
Species: Synthetic
Genetic Insert: LbCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126641 Copy
Species: Synthetic
Genetic Insert: FnoCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126642 Copy
Species: Synthetic
Genetic Insert: AsCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126640 Copy
Species: Other
Genetic Insert: Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Other, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_119710 Copy
Species: Other
Genetic Insert: Terminator paf
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Other, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_119714 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pNG2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_119893 Copy
Species:
Genetic Insert: Biotin ligase
Vector Backbone Description: Vector Backbone:pLEMO; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_119817 Copy
Species: Other
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_120230 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_120228 Copy
Species: Synthetic
Genetic Insert: SpCas9-VQR
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The TetR protein coding sequence contains an F178S difference compared to the reference sequence that does not impact the function of the plasmid.
Proper citation: RRID:Addgene_120224 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_120225 Copy
Species: Other
Genetic Insert: Avs4 chimera
Vector Backbone Description: Backbone Size:2334; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_188802 Copy
Species:
Genetic Insert: Opto-T7RNAP*(69), half expr.
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101669 Copy
Species:
Genetic Insert: Opto-T7RNAP*(69), equal expr.
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101670 Copy
Species:
Genetic Insert: Opto-T7RNAP*(563-F1), equal expr.
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101671 Copy
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