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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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XE66 Activin Betab in Cs2+ Resource Report Resource Website |
RRID:Addgene_17012 | Activin Beta B | Xenopus laevis | Ampicillin | PMID: | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:23 | 0 | ||
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XE25 Dominant Negative Activin Receptor Resource Report Resource Website |
RRID:Addgene_17005 | DN Activin Receptor | Xenopus laevis | Ampicillin | PMID: | Insert: Sac II, blunt ended fragment. Transcription: linearize with EcoR1 and transcribe with SP6. Plasmid History: Constructed by Ali Hemmati-Brivanlou on 2/14/91. For expression in embryos. Note that this construct does not have any 5' untranslated sequences and has lost the Bam H1 sits from the BS polylinker. (other names delta-1XAR1) | Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | Dominant negative | 2022-04-22 03:32:22 | 0 |
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XE185 Xenopus Delta-CS2 Resource Report Resource Website |
RRID:Addgene_17036 | Delta | Xenopus laevis | Ampicillin | PMID: | The full coding sequence of Xenopus Delta is present in this construct. | Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:28 | 0 | |
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M13 pCSKA-CAT Resource Report Resource Website |
RRID:Addgene_17150 | Cytoskeletal actin promoter | Xenopus laevis | Ampicillin | PMID: | Plasmid History: From: Richard Harland. Notes for Use: Description: Cytoskeletal actin promoter driving expression of CAT. | Backbone Size:0; Vector Backbone:pCSKA-CAT; Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:32:48 | 0 | |
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pCS2+-wbp2nlF127P Resource Report Resource Website |
RRID:Addgene_174507 | wbp2nlF127P | Xenopus laevis | Ampicillin | PMID:28663133 | we cloned this plasmid | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin | changed Phenylalanine at aa127 to Proline | 2022-04-22 03:33:22 | 0 |
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C245 Resource Report Resource Website |
RRID:Addgene_174351 | rpl3.L | Xenopus laevis | Ampicillin | PMID:34213414 | The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length. | Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin | 2022-04-22 03:33:20 | 0 | |
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C064 Resource Report Resource Website |
RRID:Addgene_174353 | PABPC1 | Xenopus laevis | Kanamycin | PMID:34213414 | Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:33:20 | 0 | ||
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C078 Resource Report Resource Website |
RRID:Addgene_174347 | ePAB | Xenopus laevis | Ampicillin | PMID:34213414 | The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length. | Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin | 13 silent mutations: G243A, A273G, G339A, C345G, G870C, A1134T, G1155A, C1173G, C1279T, C1284T, T1332C, A1434G, C1536T, C1569T | 2022-04-22 03:33:19 | 0 |
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C077 Resource Report Resource Website |
RRID:Addgene_174346 | PABPC1 | Xenopus laevis | Ampicillin | PMID:34213414 | The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length. | Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin | 2022-04-22 03:33:19 | 0 | |
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xfz8N/pCS2+ Resource Report Resource Website |
RRID:Addgene_16322 | frizzled-8 | Xenopus laevis | Ampicillin | PMID:9636083 | This construct contains the amino terminus of xfz8, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. This plasmid does not contain a myc tag and is potent at causing enlarged cement gland and shortened axes. This construct was generated by removing a ClaI-XhoI fragment from Nxfz8/CS2MT. For sense transcripts, cut with NotI. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Extracellular ligand binding domain of frizzled-8. Secreted wnt inhibitor. | 2022-04-22 03:30:49 | 0 |
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GSK3beta/pET29b Resource Report Resource Website |
RRID:Addgene_16315 | GSK3beta | Xenopus laevis | Kanamycin | PMID:9169052 | Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:30:48 | 0 | ||
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xfz2/pCS2+ Resource Report Resource Website |
RRID:Addgene_16311 | frizzled-2 | Xenopus laevis | Ampicillin | PMID:10495293 | For sense transcripts, cut with NotI and transcribe with SP6. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:47 | 0 | |
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xfz3N/pCS2+ Resource Report Resource Website |
RRID:Addgene_16328 | frizzled-3 | Xenopus laevis | Ampicillin | PMID:11585792 | This construct contains the amino terminus of xfz3, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. For sense transcripts, cut with NotI and transcribe with SP6. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Extracellular ligand binding domain of Xenopus frizzled-3. | 2022-04-22 03:30:50 | 0 |
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xfz7N/pCS2+ Resource Report Resource Website |
RRID:Addgene_16325 | frizzled-7 | Xenopus laevis | Ampicillin | PMID:9636083 | Contains xfz7 cysteine rich extracellular domain, up to 1st TM. This construct was made from Nxfz7myc. The myc tag was removed. The insert has a BamHI site at the 5' end. The myc tag was removed as a ClaI-XbaI fragment and the ends were blunted, so there are no 3' sites to cut it out. For sense transcripts, cut with NotI. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | Amino terminal end | 2022-04-22 03:30:50 | 0 |
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xfz8 probe/pBS Resource Report Resource Website |
RRID:Addgene_16323 | frizzled-8 | Xenopus laevis | Ampicillin | PMID:9636083 | Contains bases 1159-3240 of xfz8 cDNA, from just after TM2 to polyA. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Base pairs 1159-3240 of xfz8 cDNA | 2022-04-22 03:30:49 | 0 |
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GSK-3beta S9A (Xenopus)/pCS2+ Resource Report Resource Website |
RRID:Addgene_16333 | GSK-3beta | Xenopus laevis | Ampicillin | PMID: | Serine to alanine mutation (s9a) made in Xenopus GSK-3beta originally from Kimelman lab's XG73. For sense transcripts cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | GSK-3 with serine-9 to alanine mutation | 2022-04-22 03:30:52 | 0 |
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Axin GID 1-2/pCS2MT Resource Report Resource Website |
RRID:Addgene_16299 | Axin GID 1-2 | Xenopus laevis | Ampicillin | PMID:10490650 | For sense transcripts, cut with NotI and transcribe with SP6. Potent GSK-3 inhibitor and Wnt activator. | Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | GSK interaction domain aa 320-429 | 2022-04-22 03:30:45 | 0 |
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Axin GID 1-2 LP/pCS2MT Resource Report Resource Website |
RRID:Addgene_16300 | Axin GID 1-2 LP | Xenopus laevis | Ampicillin | PMID:10490650 | L->P version has a single base pair change converting leu to pro. This mutant does not bind or inhibit GSK-3beta, does not activate LEF/tcf reporters, and does not dorsalize embryos. Based on Smalley et al (EMBO J). For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | GSK-3 interaction domain aa 320-429 with L to P mutation | 2022-04-22 03:30:45 | 0 |
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kermit/pCS2+ Resource Report Resource Website |
RRID:Addgene_16309 | Kermit | Xenopus laevis | Ampicillin | PMID:11585793 | For sense transcripts, cut with NotI and transcribe with SP6. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:30:47 | 0 | |
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Axin AID/pCS2HA Resource Report Resource Website |
RRID:Addgene_16308 | Axin Axin Interaction Domain | Xenopus laevis | Ampicillin | PMID:10490650 | For sense transcripts, cut with NotI and transcribe with SP6 | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin | AID (Axin interaction domain): aa 510-777 | 2022-04-22 03:30:47 | 0 |
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