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Species: Homo sapiens
Genetic Insert: BirA*-3xFLAG-CSE1L
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TT 3xFLAG-BirA w ccdB; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192312 Copy
Species: Other
Genetic Insert: BirA*
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TT 3xFLAG-BirA; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192310 Copy
Species: Homo sapiens
Genetic Insert: TWIST2
Vector Backbone Description: Backbone Marker:Addgene #2752; Vector Backbone:pEN_TTmiRC2; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_197935 Copy
Species: Homo sapiens
Genetic Insert: NUP37
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192299 Copy
Species: Homo sapiens
Genetic Insert: BCAS2
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192294 Copy
Species: Homo sapiens
Genetic Insert: DYNLL2
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_192296 Copy
Species: Synthetic
Genetic Insert: Full-length engineered CD64, derived from human CD64
Vector Backbone Description: Backbone Marker:backbone derived from pACEBac1, Geneva Biotech; Vector Backbone:pGS_C_Strep, Addgene no. 101652; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_109191 Copy
Species: Other
Genetic Insert: ShTnsB, ShTnsC, ShTniQ, ShCas12k
Vector Backbone Description: Backbone Size:3359; Vector Backbone:pR01600; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: The vector backbone was derived from plasmid pFlpe2 from
Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07
Proper citation: RRID:Addgene_180076 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2545; Vector Backbone:BASIC_SEVA_69.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_194214 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments: Replicating ORBIT V1 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205291 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pVS1/p15a; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the XbaI site.
Proper citation: RRID:Addgene_37814 Copy
Species: jellyfish
Genetic Insert: gfpmut 3.1
Vector Backbone Description: Backbone Size:4768; Vector Backbone:pBBRMCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank accession number: JQ895027
pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame.
This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites
Proper citation: RRID:Addgene_40084 Copy
Species: Homo sapiens
Genetic Insert: Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
Vector Backbone Description: Vector Backbone:pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_134204 Copy
Species: Synthetic
Genetic Insert: Citrine-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3374; Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_44586 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + YFP Venus
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Other, cloning vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_54411 Copy
Species: Synthetic
Genetic Insert: FLAG tag + and short linker + superfolder GFP 11 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Other, cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54430 Copy
Species: Synthetic
Genetic Insert: 16 aa GS linker + superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:Other, cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54452 Copy
Species: Synthetic
Genetic Insert: superfolder GFP 1-10 domain
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:Other, cloning vector; Bacterial Resistance:Gentamicin
References:
Comments: Please note this plasmid contains super-folder GFP and shows noticeable background fluorescence.
Proper citation: RRID:Addgene_54461 Copy
Species: Mus musculus
Genetic Insert: BICD2N (1-400)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pOmniBac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_64070 Copy
Species:
Genetic Insert: ecfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments: GenBank Accession # DQ493878
Proper citation: RRID:Addgene_65030 Copy
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