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Species: Xenopus laevis
Genetic Insert: Activin Beta B
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17012 Copy
Species: Xenopus laevis
Genetic Insert: DN Activin Receptor
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: Sac II, blunt ended fragment. Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Ali Hemmati-Brivanlou on 2/14/91.
For expression in embryos. Note that this construct does not have any 5' untranslated sequences and has lost the Bam H1 sits from the BS polylinker. (other names delta-1XAR1)
Proper citation: RRID:Addgene_17005 Copy
Species: Xenopus laevis
Genetic Insert: Delta
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: The full coding sequence of Xenopus Delta is present in this construct.
Proper citation: RRID:Addgene_17036 Copy
Species: Xenopus laevis
Genetic Insert: Cytoskeletal actin promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCSKA-CAT; Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History:
From: Richard Harland.
Notes for Use:
Description: Cytoskeletal actin promoter driving expression of CAT.
Proper citation: RRID:Addgene_17150 Copy
Species: Xenopus laevis
Genetic Insert: wbp2nlF127P
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: we cloned this plasmid
Proper citation: RRID:Addgene_174507 Copy
Species: Xenopus laevis
Genetic Insert: rpl3.L
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin
References:
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174351 Copy
Species: Xenopus laevis
Genetic Insert: PABPC1
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_174353 Copy
Species: Xenopus laevis
Genetic Insert: ePAB
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin
References:
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174347 Copy
Species: Xenopus laevis
Genetic Insert: PABPC1
Vector Backbone Description: Vector Backbone:pGEM_11Zf; Vector Types:Other, in vitro transcription template; Bacterial Resistance:Ampicillin
References:
Comments: The absolute length of the poly(A) region may not be accurate. The poly(A) region may not be completely maintained during amplification from bacteria. Single clones need to be screened to verify poly(A) length.
Proper citation: RRID:Addgene_174346 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-8
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: This construct contains the amino terminus of xfz8, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. This plasmid does not contain a myc tag and is potent at causing enlarged cement gland and shortened axes. This construct was generated by removing a ClaI-XhoI fragment from Nxfz8/CS2MT. For sense transcripts, cut with NotI.
Proper citation: RRID:Addgene_16322 Copy
Species: Xenopus laevis
Genetic Insert: GSK3beta
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_16315 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6.
Proper citation: RRID:Addgene_16311 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-3
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: This construct contains the amino terminus of xfz3, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region.
For sense transcripts, cut with NotI and transcribe with SP6.
Proper citation: RRID:Addgene_16328 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: Contains xfz7 cysteine rich extracellular domain, up to 1st TM. This construct was made from Nxfz7myc. The myc tag was removed. The insert has a BamHI site at the 5' end. The myc tag was removed as a ClaI-XbaI fragment and the ends were blunted, so there are no 3' sites to cut it out. For sense transcripts, cut with NotI.
Proper citation: RRID:Addgene_16325 Copy
Species: Xenopus laevis
Genetic Insert: frizzled-8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Contains bases 1159-3240 of xfz8 cDNA, from just after TM2 to polyA.
Proper citation: RRID:Addgene_16323 Copy
Species: Xenopus laevis
Genetic Insert: GSK-3beta
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: Serine to alanine mutation (s9a) made in Xenopus GSK-3beta originally from Kimelman lab's XG73. For sense transcripts cut with NotI and transcribe with SP6
Proper citation: RRID:Addgene_16333 Copy
Species: Xenopus laevis
Genetic Insert: Axin GID 1-2
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6.
Potent GSK-3 inhibitor and Wnt activator.
Proper citation: RRID:Addgene_16299 Copy
Species: Xenopus laevis
Genetic Insert: Axin GID 1-2 LP
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: L->P version has a single base pair change converting leu to pro. This mutant does not bind or inhibit GSK-3beta, does not activate LEF/tcf reporters, and does not dorsalize embryos. Based on Smalley et al (EMBO J). For sense transcripts, cut with NotI and transcribe with SP6
Proper citation: RRID:Addgene_16300 Copy
Species: Xenopus laevis
Genetic Insert: Kermit
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6.
Proper citation: RRID:Addgene_16309 Copy
Species: Xenopus laevis
Genetic Insert: Axin Axin Interaction Domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense transcripts, cut with NotI and transcribe with SP6
Proper citation: RRID:Addgene_16308 Copy
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