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Species: Other
Genetic Insert: Rep2/Cap2
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104963 Copy
Species: Other
Genetic Insert: SENP1
Vector Backbone Description: Vector Backbone:pQE80 derivative; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_104962 Copy
Species: Other
Genetic Insert: Rep2/Cap5
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104964 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AMPK-EV
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105241 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core number p1302
Proper citation: RRID:Addgene_105538 Copy
Species: Other
Genetic Insert: CB7-CI-mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core number p1969
Proper citation: RRID:Addgene_105544 Copy
Species: Other
Genetic Insert: TurboRFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core number p2642
Proper citation: RRID:Addgene_105552 Copy
Species: Synthetic
Genetic Insert: stGtACR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105669 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5609; Vector Backbone:pAAV-Ef1a-fDIO hChR2(H134R)-EYFP; Vector Types:Mammalian Expression, AAV, Other, Flp/Frt; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105714 Copy
Species: Homo sapiens
Genetic Insert: ZNF598
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5059; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105690 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, Other, Flp-In competent; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has found the VN173 mutation (I152L) is not present in this plasmid.
This vector is a member of the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101). pKK-BiFC-Venus vector encodes fragments of Venus (VN173, VC155) that become fluorescent when they reassemble into full fluorescent protein. Thus, it is useful for protein-protein interactions studies involving bimolecular fluorescence complementation approach (BiFC) (PMID: 16454041, 18846096). Here, an improved version of VN173 fragment is used (I152L) to inhibit self-assembly (PMID:21091444). Venus fragments coding sequences were cloned from Addgene ID: 22010 and 22011 and VN173 was subjected to site-directed mutagenesis to introduce mutation I152L.
Proper citation: RRID:Addgene_105804 Copy
Species: Homo sapiens
Genetic Insert: dynactin1-811
Vector Backbone Description: Vector Backbone:GWpTolDest(R4-R2)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The depositor notes that the L287M found in dynactin is not of functional concern.
Proper citation: RRID:Addgene_105970 Copy
Species: Homo sapiens
Genetic Insert: Stathmin1
Vector Backbone Description: Vector Backbone:GWpTol2Dest(R4-R3)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_105969 Copy
Species: Other
Genetic Insert: PspCas13b
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Benchling link: https://benchling.com/s/seq-HASFia3255bkdC9iUtxu
Proper citation: RRID:Addgene_103869 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_107580 Copy
Species:
Genetic Insert: pCDNA3.1hygro-BamHI-CLuc1433-NotI
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107611 Copy
Species:
Genetic Insert: NLucYAP15
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system.
Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind.
Proper citation: RRID:Addgene_107610 Copy
Species:
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as AV-9-PV4365 from the Penn Vector Core. GCaMP6s was created as part of the GENIE project at Janelia Research Campus.
Proper citation: RRID:Addgene_107790 Copy
Species:
Genetic Insert: Ag43
Vector Backbone Description: Backbone Marker:Andreas Moeglich Lab; Backbone Size:7293; Vector Backbone:pDawn; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_107742 Copy
Species: Synthetic
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pAL; Vector Types:Worm Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_107745 Copy
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