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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAAV2/2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_104963 | Rep2/Cap2 | Other | Ampicillin | PMID: | Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields. | Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:52 | 1 | |
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pSF1389 Resource Report Resource Website 1+ mentions |
RRID:Addgene_104962 | SENP1 | Other | Kanamycin | PMID:24636565 | Vector Backbone:pQE80 derivative; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:52 | 1 | ||
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pAAV2/5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_104964 | Rep2/Cap5 | Other | Ampicillin | PMID: | Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields. | Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:52 | 1 | |
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pPBbsr2-4031NES Resource Report Resource Website 1+ mentions |
RRID:Addgene_105241 | AMPK-EV | Saccharomyces cerevisiae | Ampicillin | PMID:29186696 | Backbone Size:6800; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:56 | 1 | ||
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pENN.AAV.TBG.PI.ffLuciferase.RBG Resource Report Resource Website 1+ mentions |
RRID:Addgene_105538 | Luciferase | Other | Ampicillin | PMID: | Penn Vector Core number p1302 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:01 | 1 | |
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pENN.AAV.CB7.CI.mCherry.WPRE.RBG Resource Report Resource Website 1+ mentions |
RRID:Addgene_105544 | CB7-CI-mCherry | Other | Ampicillin | PMID: | Penn Vector Core number p1969 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:01 | 1 | |
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pENN.AAV.hSyn.TurboRFP.WPRE.RBG Resource Report Resource Website 1+ mentions |
RRID:Addgene_105552 | TurboRFP | Other | Ampicillin | PMID: | Penn Vector Core number p2642 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:02 | 1 | |
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pAAV-CKIIa-stGtACR2-FusionRed Resource Report Resource Website 1+ mentions |
RRID:Addgene_105669 | stGtACR2 | Synthetic | Ampicillin | PMID:30297821 | Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:03 | 2 | ||
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pAAV-Ef1a-fDIO-GCaMP6s Resource Report Resource Website 1+ mentions |
RRID:Addgene_105714 | GCaMP6s | Rattus norvegicus | Ampicillin | PMID: | Backbone Marker:Karl Deisseroth; Backbone Size:5609; Vector Backbone:pAAV-Ef1a-fDIO hChR2(H134R)-EYFP; Vector Types:Mammalian Expression, AAV, Other, Flp/Frt; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:04 | 1 | ||
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pcDNA3.1-ZNF598-TEV-3xFLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_105690 | ZNF598 | Homo sapiens | Ampicillin | PMID:28065601 | Backbone Marker:Invitrogen; Backbone Size:5059; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:04 | 1 | ||
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pKK-BiFC-Venus Resource Report Resource Website 1+ mentions |
RRID:Addgene_105804 | Ampicillin | PMID:29590189 | Addgene has found the VN173 mutation (I152L) is not present in this plasmid. This vector is a member of the pKK vector family. It enables concomitant expression of two genes, which transcription is driven by a tetracycline responsive bidirectional CMV promoter. This vector is a derivate of the BI16 plasmid (PMID: 16212928) and is suitable for stable cell line generation using the Flp-In system (see Szczesny RJ et al. for detailed description and protocols; bioRxiv, doi: https://doi.org/10.1101/160101). pKK-BiFC-Venus vector encodes fragments of Venus (VN173, VC155) that become fluorescent when they reassemble into full fluorescent protein. Thus, it is useful for protein-protein interactions studies involving bimolecular fluorescence complementation approach (BiFC) (PMID: 16454041, 18846096). Here, an improved version of VN173 fragment is used (I152L) to inhibit self-assembly (PMID:21091444). Venus fragments coding sequences were cloned from Addgene ID: 22010 and 22011 and VN173 was subjected to site-directed mutagenesis to introduce mutation I152L. | Backbone Marker:Sammarco & Grabczyk (PMID: 16212928); Vector Backbone:BI-16 (constructed based on pcDNA5/FRT/TO from Invitrogen); Vector Types:Mammalian Expression, Other, Flp-In competent; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:06 | 1 | |||
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hsp70: mKate2 dynactin1-811 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105970 | dynactin1-811 | Homo sapiens | Ampicillin | PMID:26879757 | The depositor notes that the L287M found in dynactin is not of functional concern. | Vector Backbone:GWpTolDest(R4-R2)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | contains only amino acids 1-811 | 2022-04-22 03:16:08 | 1 |
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hsp70:Stathmin1-mKate2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105969 | Stathmin1 | Homo sapiens | Ampicillin | PMID:26879757 | Vector Backbone:GWpTol2Dest(R4-R3)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:08 | 1 | ||
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pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090 Resource Report Resource Website 1+ mentions |
RRID:Addgene_103869 | PspCas13b | Other | Ampicillin | PMID:29070703 | Benchling link: https://benchling.com/s/seq-HASFia3255bkdC9iUtxu | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity. Lacks residues 984-1090. | 2022-04-22 03:15:39 | 1 |
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AICSDP-42:AAVS1-mTagRFPT-CAAX Resource Report Resource Website 1+ mentions |
RRID:Addgene_107580 | AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:16:40 | 1 |
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pCDNA3.1neo-14-3-3-CLuc Resource Report Resource Website 1+ mentions |
RRID:Addgene_107611 | pCDNA3.1hygro-BamHI-CLuc1433-NotI | Ampicillin | PMID:29535383 | To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system. Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind. | Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:41 | 1 | ||
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pCDNA3.1neo-NLucYAP15 Resource Report Resource Website 1+ mentions |
RRID:Addgene_107610 | NLucYAP15 | Ampicillin | PMID:29535383 | To get a good signal this biosensor should be used fresh and pcDNA3.1-MST1-myc might be used as a positive control for the system. Because of feedback pathways, signaling pathways that increase YAP/TAZ activity sometimes also finally increase LATS and LATS biosensor activity. So, to interpret biosensor signals it is good to always keep the effect of feedback signaling in mind. | Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:41 | 2 | ||
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AAV.CamKII.GCaMP6s.WPRE.SV40 Resource Report Resource Website 1+ mentions |
RRID:Addgene_107790 | GCaMP6s | Ampicillin | PMID: | This plasmid was previously available as AV-9-PV4365 from the Penn Vector Core. GCaMP6s was created as part of the GENIE project at Janelia Research Campus. | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:43 | 1 | ||
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pDawn-Ag43 Resource Report Resource Website 1+ mentions |
RRID:Addgene_107742 | Ag43 | Spectinomycin | PMID:29555779 | Ag43 contains a G981S variant compared to the NCBI reference (WP_000820410.1); plasmid functions as described in the associated publication. | Backbone Marker:Andreas Moeglich Lab; Backbone Size:7293; Vector Backbone:pDawn; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | removed all pstI cut sites with silent mutations, added RBS | 2022-04-22 03:16:43 | 1 | |
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pmec-4::Chrimson::SL2::mCherry::unc-54 Resource Report Resource Website 1+ mentions |
RRID:Addgene_107745 | Chrimson | Synthetic | Ampicillin | PMID:29943731 | Vector Backbone:pAL; Vector Types:Worm Expression, Insect Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:16:43 | 1 |
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