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Species: Other
Genetic Insert: gfp, hyg
Vector Backbone Description: Vector Backbone:pYS2; Vector Types:Other, Deletion generation; Bacterial Resistance:Hygromycin
References:
Comments: Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117490 Copy
Species: Other
Genetic Insert: Htb2-GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments: Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117669 Copy
Species: Synthetic
Genetic Insert: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134316 Copy
Species:
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_119884 Copy
Species:
Genetic Insert: GFP(47TAG)
Vector Backbone Description: Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed.
Proper citation: RRID:Addgene_193251 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Matthew Bennett; Backbone Size:5611; Vector Backbone:pYUBcLIC-GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: pYUB vector backbone originates from pYUB28b (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277).
Reference: Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. doi: 10.1371/journal.pone.0015803 PubMed 21209917
****
LIC cassette together with folding reporter GFP and purification tag sequence originate from pREcLIC-GFP.
Reference: Geertsma, E., Poolman, B. High-throughput cloning and expression in recalcitrant bacteria. Nat Methods 4, 705–707 (2007). doi: 10.1038/nmeth1073 PubMed 17643108
Proper citation: RRID:Addgene_183509 Copy
Species:
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Chunbo Lou; Backbone Size:6468; Vector Backbone:pTHS; Vector Types:CRISPR, Other, Integrative vector for use in Streptomyces; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_210387 Copy
Species:
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_157877 Copy
Species: Other
Genetic Insert: gRNA1 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:Other; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_118078 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_118077 Copy
Species: Other
Genetic Insert: gRNA2 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:Other; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_118079 Copy
Species: Other
Genetic Insert: pTPI1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_118075 Copy
Species: Other
Genetic Insert: pGAP - nnH3H_v2 - tAOX
Vector Backbone Description: Backbone Size:4113; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_219752 Copy
Species: Other
Genetic Insert: pGAP - npgA - tAOX
Vector Backbone Description: Backbone Size:3503; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_219750 Copy
Species:
Genetic Insert: none, cloning vector for introduction of targeting sequences
Vector Backbone Description: Vector Backbone:pCHERRY3 : pAL5000/colE1 origins; Vector Types:CRISPR; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_221387 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.
Proper citation: RRID:Addgene_222351 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9662; Vector Backbone:pBAC2015, pQS-gusA, and pUC19; Vector Types:Other; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_227508 Copy
Species:
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_227582 Copy
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