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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pYS2 Resource Report Resource Website |
RRID:Addgene_117490 | gfp, hyg | Other | Hygromycin | PMID:24100088 | Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication. | Vector Backbone:pYS2; Vector Types:Other, Deletion generation; Bacterial Resistance:Hygromycin | 2023-09-15 01:02:31 | 0 | |
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Htb2-GFP Resource Report Resource Website |
RRID:Addgene_117669 | Htb2-GFP | Other | Hygromycin | PMID:30314480 | Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function. | Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | Contains the gene encoding the Histone Htb2 from S.cerevisiae tagged with a GFP at the C-terminal. The protein is expressed constituvely by the promoter pGAP. | 2023-09-15 01:02:32 | 0 |
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pSiMPlh_N + pSiMPlh_C Resource Report Resource Website |
RRID:Addgene_134316 | hygR_gp41-1 N-intein + hygR_gp41-1 C-intein | Synthetic | Hygromycin | PMID:31672972 | pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. | Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | P254Q in hygR_gp41-1 N-intein | 2023-09-15 01:03:46 | 0 |
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pDD108 Resource Report Resource Website |
RRID:Addgene_119884 | BbaJ23104_EYFP | Hygromycin | PMID:29366319 | Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2023-09-15 01:02:54 | 0 | |||
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pSMT-MjtRNA-GFP(47TAG) Resource Report Resource Website |
RRID:Addgene_193251 | GFP(47TAG) | Hygromycin | PMID:28276676 | Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed. | Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | Codon for Tyr47 replaced with amber stop codon TAG (nucleotide position 151). | 2023-09-15 01:09:24 | 0 | |
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pYUBcLIC-GFP Resource Report Resource Website |
RRID:Addgene_183509 | Hygromycin | PMID:35750296 | pYUB vector backbone originates from pYUB28b (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277). Reference: Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. doi: 10.1371/journal.pone.0015803 PubMed 21209917 **** LIC cassette together with folding reporter GFP and purification tag sequence originate from pREcLIC-GFP. Reference: Geertsma, E., Poolman, B. High-throughput cloning and expression in recalcitrant bacteria. Nat Methods 4, 705–707 (2007). doi: 10.1038/nmeth1073 PubMed 17643108 | Backbone Marker:Matthew Bennett; Backbone Size:5611; Vector Backbone:pYUBcLIC-GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2023-09-15 01:08:00 | 0 | |||
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pCB-1 Resource Report Resource Website |
RRID:Addgene_210387 | dCas9 | Hygromycin | PMID:37801665 | Backbone Marker:Chunbo Lou; Backbone Size:6468; Vector Backbone:pTHS; Vector Types:CRISPR, Other, Integrative vector for use in Streptomyces; Bacterial Resistance:Hygromycin | The BsaI sites were removed via synonymous mutations | 2024-01-30 12:17:52 | 0 | ||
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pJDC124 Resource Report Resource Website |
RRID:Addgene_157877 | mCherry | Hygromycin | PMID:26934495 | Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2024-08-01 01:03:29 | 0 | |||
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pMST-710 Resource Report Resource Website |
RRID:Addgene_118078 | gRNA1 (CexA) | Other | Hygromycin | PMID:30553933 | Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:Other; Bacterial Resistance:Hygromycin | 2024-02-27 12:01:08 | 0 | ||
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BB3_arscen_URA3_Hygro Resource Report Resource Website |
RRID:Addgene_118077 | Hygromycin | PMID:30553933 | Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | 2024-02-27 12:01:08 | 0 | ||||
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pMST-711 Resource Report Resource Website |
RRID:Addgene_118079 | gRNA2 (CexA) | Other | Hygromycin | PMID:30553933 | Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:Other; Bacterial Resistance:Hygromycin | 2024-02-27 12:01:08 | 0 | ||
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pMST-1312 Resource Report Resource Website |
RRID:Addgene_118075 | pTPI1-cexA-tCYC1 | Other | Hygromycin | PMID:30553933 | Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | 2024-02-27 12:01:08 | 0 | ||
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pNK5712 Resource Report Resource Website |
RRID:Addgene_219752 | pGAP - nnH3H_v2 - tAOX | Other | Hygromycin | PMID:38253843 | Backbone Size:4113; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | 2024-08-01 01:07:10 | 0 | ||
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pNK1508 Resource Report Resource Website |
RRID:Addgene_219750 | pGAP - npgA - tAOX | Other | Hygromycin | PMID:38253843 | Backbone Size:3503; Vector Backbone:Level 1-like; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin | 2024-08-01 01:07:10 | 0 | ||
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pDN-Cherry-sgRNA Resource Report Resource Website |
RRID:Addgene_221387 | none, cloning vector for introduction of targeting sequences | Hygromycin | PMID:38319218 | Vector Backbone:pCHERRY3 : pAL5000/colE1 origins; Vector Types:CRISPR; Bacterial Resistance:Hygromycin | 2024-08-01 01:07:24 | 0 | |||
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pJMP3665 Resource Report Resource Website |
RRID:Addgene_222351 | Hygromycin | PMID: | Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B. | Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin | 2024-08-01 01:07:29 | 0 | |||
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pCap-101-Hgy Resource Report Resource Website |
RRID:Addgene_227508 | Hygromycin | PMID:39666857 | Backbone Size:9662; Vector Backbone:pBAC2015, pQS-gusA, and pUC19; Vector Types:Other; Bacterial Resistance:Hygromycin | 2024-12-18 04:08:13 | 0 | ||||
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pRel-Hyg Resource Report Resource Website |
RRID:Addgene_227582 | Cas9 | Hygromycin | PMID:39666857 | Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Hygromycin | 2024-12-18 04:08:13 | 0 |
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