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URL: http://www.wormbase.org/db/get?name=WBStrain00000127
Proper Citation: RRID:WB-STRAIN:WBStrain00000127
Description: Caenorhabditis elegans with name unc-119(ed3) III; niDf199 IV; armEx58. from WB.
Species: Caenorhabditis elegans
Synonyms: unc-119(ed3) III; niDf199 IV; armEx58.
Notes: armEx58 [WRM0611aH08-Del8mer + unc-119(+)]. Pick non-Unc to maintain. This strain contains a transgenic array that expresses a derivative WRM0611aH08 fosmid. The WRM0611aH08 fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. This derivative fosmid construct lacks the upstream 8-mer motif (CTGTTTCA) next to 21U-3372. The expression of this individual 21U-RNA is lost in transgenic animals. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45.|"Made_by: Cecere & Mansisidore"
Affected Gene: WBGene00006843(unc-119)
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Source: Integrated Animals
Source Database: WormBase (WB)