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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD02201
Description: Tetrahymena thermophila with name YN-HTAX (3) from TSC.
Species: Tetrahymena thermophila
Notes: Shot Xiaoyuan Song’s i∆HTAY strain(partial replacement of HTAY with neo3) with construct MTT1-2HA tags after the ATG of HTAY n-terminus (AA1-258) followed by HTAX AA1-138) also has neo2 in 5’ flank of MTT1.Single cell line (3D) with most or all of HTAY replaced by the HA tagged YN-HTAX. Pushed to 30mg/ml pm resistance with Cd at 0.1-.0.5 ug/ml needed to express the YN-HTAX From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: HTAY (TTHERM_01079200) and HTA1 (TTHERM_00790790)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center
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