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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD01811
Description: Tetrahymena thermophila with name BMCG- ∆TWI11-5 from TSC.
Species: Tetrahymena thermophila
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center
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