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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD01741
Description: Tetrahymena thermophila with name VI006 from TSC.
Species: Tetrahymena thermophila
Notes: The knockout also deletes 213bp of the 5' flanking region and introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena. Submitted by Robert Coyne, J. Craig Venter Institute
Affected Gene: ADA2 (TTHERM_00790730)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center
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