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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD01342
Description: Tetrahymena thermophila with name cTTMG-HA #15 from TSC.
Species: Tetrahymena thermophila
Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with HA tag .Progeny of GTUKO5x6, transformed with pTTMGHA construct (GTU coding, HA tag driven by MTT promoter) to rescue GTU KO heterokaryon. Can mate with cTTMG-HA #12 or #14. Requires Cd to grow, use .25 to 1 ug/ml. GTU1-HA detected at protein level. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: GTU1 (TTHERM_00079520)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center