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Northwestern University Mouse Histology and Phenotyping Laboratory Core Facility (RRID:SCR_017870)Copy Citation Copied
URL: http://mhpl.facilities.northwestern.edu/
Proper Citation: Northwestern University Mouse Histology and Phenotyping Laboratory Core Facility (RRID:SCR_017870)
Description: Core provides assistance with standard and customized research specific histology services for variety of tissues (i.e., mouse, rat, sheep, pig, zebrafish, etc.) for routine histology, and immunohistochemistry. Provides Pathologist consultation to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding biologic actions of targeted molecule(s) or toxicity of exogenously administered substances.Provides training opportunities for learning histology techniques and phenotyping analysis.Services include Immunohistochemistry (IHC) . Using tyramide signal amplification (TSA) and ABC methods (automated), with DAB chromogenic substrate; Immunofluorescence (IF)- Single and multiple staining;TUNEL assay; Freezing and embedding of tissues for histology;Frozen sections;Paraffin block processing and Paraffin sections;Hematoxylin and Eosin staining of sections;Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains);Dissection and tissue collection;Histopathology (slide interpretation);Histopathology toxicity evaluation;Training in necropsy techniques including perfusion of mice and special tissue dissection;Assistance with development of animal pathology protocols.
Abbreviations: MHPL
Synonyms: Mouse Histology and Phenotyping Laboratory
Resource Type: service resource, core facility, access service resource
Keywords: Mouse, histology, phenotyping, tissue, rat, sheep, pig, zebrafish, immunohistochemistry, pathology, consultation, target, molecule, toxicity, substance, training, analysis, immunofluorescence, TUNEL, assay, freezing, embedding, paraffin, block, processing, section, necropsy, service, core
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