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Nebraska-Lincoln University Metabolomics and Proteomics Core Facility (RRID:SCR_017789)Copy Citation Copied
URL: https://redoxbiologycenter.unl.edu/facilities/metabolomics-and-proteomics-core-facility/
Proper Citation: Nebraska-Lincoln University Metabolomics and Proteomics Core Facility (RRID:SCR_017789)
Description: Provides tools of modern functional proteomics and metabolomics. Facility is equipped with chromatography and mass spectrometry based technologies for proteomics and metabolomics (Clinical and non clinical), personalized experimental design consultation and comprehensive, individualized bioinformatics support.Services include:Small molecule exact mass determination or quantitation using positive or negative ion mode;Protein identification using LC/MS/MS analysis and MASCOT and SEQUEST database search;Shot gun proteome analysis of biological samples;Biomarker discovery from biological fluid;Drug protein or drug nucleic acid protein interaction;Protein complex isolation and identifying interacting partners and its quantitation;Protein differential expression analysis and quantitation by 2D-LC MS/MS (MudPIT);Global PTM analysis and quantitation;Specialization in phosphorylation and oxidation analysis;Coomassie Blue and Silver Stained Gel analysis; de novo peptide sequencing by tandem mass spectrometry;Confirmation of mutations in protein ;Customized sequence search of in-house proteins that are not available in database; Post translational modifications (phosphorylation, sumoylation, ubiquitination, oxidation, etc.); Determination of oxidation state of cysteine (disulfide bonds);Intact proteins and peptides mass determination.
Synonyms: Metabolomics and Proteomics Core Facility
Resource Type: service resource, access service resource, core facility
Keywords: Metabolomics, proteomics, chromatography, mass, spectrometry, protein, peptide, analysis, service, core
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