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URL: http://www.addgene.org/79642
Proper Citation: RRID:Addgene_79642
Insert Name: NA
Bacterial Resistance: Gentamicin
Defining Citation: PMID:26829286
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:Gentamicin
Comments: If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha). Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.
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Source: Addgene
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