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URL: http://www.addgene.org/70687
Proper Citation: RRID:Addgene_70687
Insert Name: Transposon Tn5-1063
Bacterial Resistance: Other
Defining Citation: PMID:11607193
Vector Backbone Description: Backbone Marker:Wolk Lab; Vector Backbone:pRL1058; Vector Types:; Bacterial Resistance:Other
Comments: Tn5 derivative Tn5-1063a (in plasmid pRL1063a) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 and pRL1058 is this series of plasmids), providing large numbers of transposon mutants. Plasmid pJE205 bearing the luxA and luxB genes of Vibrio fischeri was the kind gift of M. Silverman, Agouron Institute, La Jolla, CA. The sequence of luxA gene in this construct has been updated and differs slightly from the original X06758.1 sequence. Additional references: Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for the synthesis of heterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Bacteriol 187: 1114-1123 Fan Q, Huang G, Lechno-Yossef S, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120. Mol Microbiol 58: 227-243
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Source: Addgene