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URL: http://www.addgene.org/61960
Proper Citation: RRID:Addgene_61960
Insert Name: none
Bacterial Resistance: None
Defining Citation: PMID:26577727
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Comments: Genotype = cysE hisG Precursor strain = YM138 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Cys, His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
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Source: Addgene
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