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Plasmid Name
pCRII_TOPO_hNEAT1
RRID:Addgene_61518 RRID Copied  
PDF Report How to cite
RRID:Addgene_61518
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Plasmid Information

URL: http://www.addgene.org/61518

Proper Citation: RRID:Addgene_61518

Insert Name: NEAT1

Organism: Homo sapiens

Bacterial Resistance: Ampicillin and Kanamycin

Defining Citation: PMID:19217333

Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4000; Vector Backbone:PCRII; Vector Types:Other, general cloning vector; Bacterial Resistance:Ampicillin and Kanamycin

Comments: *To create this plasmid, hNEAT1 was amplified from HeLa cDNA. The insert contains two mismatches (C->T at bp 78, and A->G at bp 91, an A->G at bp 1666, and a deletion of TA at bp 2031 and 2032 using the numbering of NR_028272.1) compared to the canonical hNEAT1 sequence. These variants are likely to be encoded in the HeLa genome, as they appeared in multiple clones, but this is yet to be verified by sequencing of HeLa genomic DNA.

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Data and Source Information

Source: Addgene