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URL: http://www.addgene.org/41883
Proper Citation: RRID:Addgene_41883
Insert Name: Ptac::GFP-mut-2
Organism: A. victoria
Bacterial Resistance: Hygromycin
Defining Citation: PMID:15271914
Vector Backbone Description: Backbone Size:6050; Vector Backbone:pJN105; Vector Types:Bacterial Expression, Other, Bacterial broad host range vector; Bacterial Resistance:Hygromycin
Comments: The pBBR1MCS-5 derivative of the Bordetella bronchiseptica BHR plasmid has a pBBR/BHR replicon that is maintained in L. pneumophila and is compatible with IncP, IncW, IncQ, and IncRi incompatibility groups. Plasmid pJN105 is a derivative of pBBR1MCS-5 with araC and PBAD regions before the polylinker. PCR primers with BglII sites at their 5′ ends were used to amplify the region outside the gentamicin cassette in pJN105. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206.hygro to form pJN105-hygro. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5′ ends. After digestion with NheI, this fragment was ligated with NheI-digested pJN105-hygro to form pJN105-hygro-GFP.
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Source: Addgene
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