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URL: http://www.addgene.org/40084
Proper Citation: RRID:Addgene_40084
Insert Name: gfpmut 3.1
Organism: jellyfish
Bacterial Resistance: Gentamicin
Defining Citation: PMID:22820336
Vector Backbone Description: Backbone Size:4768; Vector Backbone:pBBRMCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Comments: GenBank accession number: JQ895027 pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame. This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites
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Source: Addgene
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