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URL: http://www.addgene.org/34929
Proper Citation: RRID:Addgene_34929
Bacterial Resistance: None
Defining Citation: PMID:21868676
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:None
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.
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Source: Addgene
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