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Plasmid Name
pBCN28-R4R3
RRID:Addgene_26303 RRID Copied  
PDF Report How to cite
RRID:Addgene_26303
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Plasmid Information

URL: http://www.addgene.org/26303

Proper Citation: RRID:Addgene_26303

Bacterial Resistance: Chloramphenicol and Ampicillin

Defining Citation: PMID:20729840

Vector Backbone Description: Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin

Comments: Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans). This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

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Data and Source Information

Source: Addgene