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Plasmid Name
pEN_TmiR_G13-L_G12-E
RRID:Addgene_25763 RRID Copied  
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RRID:Addgene_25763
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Plasmid Information

URL: http://www.addgene.org/25763

Proper Citation: RRID:Addgene_25763

Insert Name: G alpha 13 and 12 miR-shRNA

Organism: Mus musculus

Bacterial Resistance: Gentamicin

Defining Citation: PMID:16945906

Vector Backbone Description: Backbone Marker:ATCC 10326362; Backbone Size:4641; Vector Backbone:pENTR1A-Gent; Vector Types:Other, Entry vector; Bacterial Resistance:Gentamicin

Comments: Entry vector with TRE-driven G alpha 12 and 13 miR-shRNA G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT G alpha 13 miR-shRNA: CTGGGTGAGTCTGTAAAGTATT There are 3 minor changes between depositor's sequence and Addgene's sequence - An extra T between the TRE promoter and first shRNA; a 17nt stretch between the shRNAs is different but will not affect function; and a single nt change mutates an EcoRI site just beyond the 2nd shRNA but will again not affect function.

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Data and Source Information

Source: Addgene