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URL: http://www.addgene.org/24682
Proper Citation: RRID:Addgene_24682
Insert Name: Chordin
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8752213
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Chordin expression vector, tagged. Insert of pSP35 Chd-Myc (Chd coding region). Insert cut from pSP35Chd-Myc (from a dcm-, dam- bacterial strain, as Xba I is there in a methylation consensus) with EcoRI and XbaI and subcloned in the same sites of pCS2. For sense RNA injection: linearize with NotI Transcribe with SP6
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Source: Addgene