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URL: http://www.addgene.org/205293
Proper Citation: RRID:Addgene_205293
Insert Name: CspRecT / Bxb-1
Organism: Synthetic
Bacterial Resistance: Gentamicin
Defining Citation: PMID:
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
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Source: Addgene