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URL: http://www.addgene.org/203682
Proper Citation: RRID:Addgene_203682
Insert Name: Yellow Fluorescent Protein (YFP)
Bacterial Resistance: Erythromycin
Defining Citation: PMID:17014739
Vector Backbone Description: Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Comments: Optimized version of the Cyan- and Yellow fluorescent proteins were created as follows: The hsp60 promoter driven GFP+ [19] was amplified and cloned into the pUC18 plasmid. pUC-yfp++ was obtained by introducing the following mutations by site-directed mutagenesis (Stratagene): F46L, S65G, V68A, S72A, S175G, T203Y, whereas the pUC-cfp++, was obtained by introducing the K26R, Y66W, N146I, N164H, S175G, N212K mutations
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Source: Addgene
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