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URL: http://www.addgene.org/17147
Proper Citation: RRID:Addgene_17147
Insert Name: NCAM promoter
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Other, Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.
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Source: Addgene
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