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URL: http://www.addgene.org/16857
Proper Citation: RRID:Addgene_16857
Insert Name: dnWnt-11
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10769246
Vector Backbone Description: Backbone Marker:Available at Addgene (#15030); Backbone Size:3300; Vector Backbone:pSP64T; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB. For injection, linearize with BamH1 and transcribe with SP6.
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Source: Addgene
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