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URL: http://www.addgene.org/16303
Proper Citation: RRID:Addgene_16303
Insert Name: Axin DeltaGID
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10490650
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Other, Vertebrate expression; Bacterial Resistance:Ampicillin
Comments: For sense transcripts, cut with NotI and transcribe with SP6. GID= GSK3 interaction domain. NcoI digest should cut out a 1.733kb insert. Please note that the GSK interaction domain deletion begins at aa 321 when the sequence is compared to GenBank entry NM_001088405.1. As a result of the cloning process, there are two amino acids 'HM' prior to the end of the deletion at aa 504 that are not part of the endogenous axin sequence. Addgene's sequencing results also found a D559E mutation in the axin sequence that reflects a single nucleotide polymorphism. The depositing laboratory states that these differences are not a concern for the function of the plasmid.
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Source: Addgene
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