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URL: http://www.addgene.org/158633
Proper Citation: RRID:Addgene_158633
Insert Name: MAVS
Organism: Homo sapiens
Bacterial Resistance: Gentamicin
Defining Citation: PMID:33740397
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Other, Gateway entry vector; Bacterial Resistance:Gentamicin
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
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Source: Addgene
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