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URL: http://www.addgene.org/15010
Proper Citation: RRID:Addgene_15010
Insert Name: activin-Vg1
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8797821
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64TS; Vector Types:Other, Sp6 expression for use in Xenopus; Bacterial Resistance:Ampicillin
Comments: Chimeric activin-Vg1 mRNA, encoding the pro-region of activin βB fused to the mature region of Vg1, resulting in the processing and secretion of mature Vg1. The activin βB-Vg1 hybrid was constructed by PCR amplification of a fragment of a Xenopus activin βB gene encoding the N-terminal pro-region, the tetrabasic cleavage site and the first four amino acids of the C-terminal mature region. This fragment was ligated, in frame, to a PCR amplified fragment of the Vg1 gene encoding the C-terminal mature region lacking the first four amino acids. The ligation product was reamplified, digested at terminal restriction sites and cloned into pSP64TS. The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site. See Author's Map for more information.
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No rating or validation information has been found for pSP64TS Activin pro Vg1 Flag (DM#171).
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Source: Addgene
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