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Plasmid Name
E. coli ER1821ΔlacI
RRID:Addgene_141407 RRID Copied  
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RRID:Addgene_141407
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Plasmid Information

URL: http://www.addgene.org/141407

Proper Citation: RRID:Addgene_141407

Bacterial Resistance: None

Defining Citation: PMID:31980824

Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None

Comments: λ- F- glnX44 e14- (McrA-) rfbD1 endA1 thi-1 Δ(yjiT-opgB)114::IS10 (EcoKI R- M- McrBC- Mrr-) + rpoS393(am) creC510 lrhA::IS3 ydeN::IS10 ΔlacI Verification of lacI deletion: PCR reaction on genomic DNA using AK362 and AK365 primers produces a 1936 bp fragment AK 362 5’-CAATACCAATCGCACGCGG AK 365 5’-CGAGACGTCACGGAAAATGCC Phenotype: Constitutive β-galactosidase synthesis This strain was derived from the precursor strain, E. coli ER1821, which is described in Jobling et al. (2016) Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems. Genome Announc 4(4):e00763-16. https://www.ncbi.nlm.nih.gov/pubmed/27516504

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Data and Source Information

Source: Addgene