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URL: http://www.addgene.org/13986
Proper Citation: RRID:Addgene_13986
Insert Name: Hoxc6
Organism: Gallus gallus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8812121
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
Comments: To generate a retrovirus capable of transcribing Hoxc-6 mRNA, the region encoding the PR11 Hoxc-6 protein was isolated from a chick cDNA clone (Nelson et al., 1996). The PR11 Hoxc-6 protein comprises 235 amino acids (Oliver et al., 1988; Coletta et al., 1991; Shimeld et al., 1993). The ATG of the PR11 coding region was engineered by PCR for cloning into the NcoI site of the SLAX-13 shuttle vector (Riddle et al., 1993), and then subcloned into the retroviral vector RCAS-BP(A) (Hughes et al., 1987). The insert is 932 base pairs and includes approximately 230 bp of 3' untranslated sequence.
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Source: Addgene
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