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URL: http://www.addgene.org/137997
Proper Citation: RRID:Addgene_137997
Insert Name: Tn5 tnp
Organism: Synthetic
Bacterial Resistance: Other
Defining Citation: PMID:32963323
Vector Backbone Description: Vector Backbone:pFG051; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Other
Comments: This plasmid was designed for high density transposon mutagenesis experiment. It can be delivered by conjugation very efficiently for it's transposon to then be inserted in the recipient DNA. The donor strain must have pFG036 as well to avoid unwanted transposition. Indeed, the promoter (pL) in front of the Tn5 transposase encoding gene is repressed by cI expressed from pFG036. The cloning strain must also have pFG036 to be able to maintain pFG051. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.
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Source: Addgene
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