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URL: http://www.addgene.org/134316
Proper Citation: RRID:Addgene_134316
Insert Name: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Organism: Synthetic
Bacterial Resistance: Hygromycin
Defining Citation: PMID:31672972
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
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Source: Addgene
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