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URL: http://www.addgene.org/13333
Proper Citation: RRID:Addgene_13333
Organism: This is a strain, not a plasmid
Bacterial Resistance: Other
Defining Citation: PMID:6309403
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Other
Comments: The strain was derived as follows: a culture of E. coli N99 galK strA was mutagenized with 1-methyl-3'-nitrosoguanidine and Cou-resistant cells were selected on LB agar containing 25ug/mL Cou and 10mM sodium pyrophosphate. These colonies were then screened for failure to grow on LB agar at 42oC. One such strain, N4175, was used as a donor for P1 transduction into N99. The transductant strain, N4177, is Cou-resistant at 32oC and is unable to grow at 42oC. GyrB protein partly purified from N4177 cells grown at 32oC, when added to wild-type GyrA protein, yields a DNA gyrase complex whose supercoiling activity is Cou-resistant at 25oC and temperature sensitive (less than 1% of the activity of the normal enzyme at 42oC).
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Source: Addgene
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