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URL: http://www.addgene.org/122017
Proper Citation: RRID:Addgene_122017
Insert Name: GGPP synthase
Organism: Other
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:30584096
Vector Backbone Description: Backbone Size:6200; Vector Backbone:psc101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: Based on pAC-LYCipi (Addgene plasmid # 53279 ; http://n2t.net/addgene:53279 ; RRID:Addgene_53279), with backbone changed to pSC101, and crtE swapped for ggpps from Taxus canadensis (truncated & codon optimized). Please note: The plasmid contains a 387 nucleotide deletion from Trp249 of Eh-idi to His30 of IDI-2 (when compared to the depositor's sequence) combining the two ORFs into a single ORF. This mutation is not known to affect plasmid function.
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Source: Addgene
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