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URL: http://www.addgene.org/102785
Proper Citation: RRID:Addgene_102785
Insert Name: Rab5a
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
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Source: Addgene
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